Investigation #9- Biotechnology- Restriction Enzyme Analysis of DNA
PLF- due Thursday BOP-
Question- how can we use genetic information to identify and profile individuals?
- cast gels
- Pippet dye
- pippet samples into gel
- run electrophoresis
- Lab Manual- Page 120: Analyzing results- steps 1-4.- Due tomorrow
Assignment- Due Tuesday Feb 24
- Big Idea 1- Essential knowledge and illustrative examples.
AP Biology- concept outline- Big Idea 1: Evolution starts on page 8- read over introduction/overview.
Go to the following link to see the assigned elements for this Big Idea
-Chapters 24/25 due Tuesday after break
- be ready to give your illustrative example Tuesday BOP
-Case study- "Tree Thinking"- deadline Moved to Thursday after break
-Lab manual- Restriction enzyme analysis of DNA: Page 123 Thinking about your results- questions 1-4- (be ready to share in class when we get back from break.
DNA Fingerprinting Lab- go over parts 1-4- see what your standard curve would look like using a semi-log graph.(one on BioRad packet)
-write up the results- (use quantitative data) for your gel and a conclusion/discussion for your gel (or another group's gel).
- Post lab (BioRad packet): Interpret results- page 46 -questions 1-7
-read extension activity on pages 47-49 and answer questions 1-8 on pages 50/51.
Great overview of DNA fingerprinting!
Lab set up- learn how to use micropipets and micro centrifuge. 10 pts.
1 page summary about the attached article on
PP File below: In-class presentation: DNA Fingerprinting